Journal of Innovation in Applied Research (eISSN: 2581-4206)

Jounal of Innovation in Applied Research
(eISSN: 2581-4206)
Isolation and Characterization of Cellulose derived from Kola Nut (Cola Sterculiaceae)Testa
Plant Biotechnology | Volume 5 (Issue 1&2)

Isolation and Characterization of Cellulose derived from Kola Nut (Cola Sterculiaceae)Testa

Agboeze E,Uchechukwu T O, Ogbobe O.
Open Access   
Published : 30-Apr-2022

DOI: 10.51323/JIAR.5.1.2022.25-38

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True cellulose in kola nut testa was isolated by the techniques of multi-stage pulping and bleaching using NaClO2 after initial delignification using NaOH as alkali. After identification by the iodated-ZnCl2 method, KT α-cellulose was evaluated for organoleptic properties and characterized according to physiochemical properties, inherent functional groups as well as surface morphology and degree of crystallinity. The results showed that 12.4% of granular, off-white, odourless, tasteless and insoluble KT α-cellulose was recovered from 150 g of extractive free KT. KT α-cellulose, characterized by a pH of 6. 4 and moisture sorption capacity of 1.8% as well as true, bulk and tap density values of 1.19, 0.29 and 0.43 gcm-3 respectively showed to contain 1.5% ash after carbonization. Fourier Transform Infra-red spectrophotometric (FTIR) analysis showed the presence of strong and broad absorption bands around 3257 cm-1 which were assigned to intermolecular and intramolecular stretching vibrations of the -OH groups in KT α-cellulose. A sharp band identified near 2919 cm-1 was ascribed to the asymmetric and symmetric stretches of the CH2 and CH bonds in the biopolymer. The scanning electron micrograph (SEM) of KT a-cellulose revealed that the biopolymer consists of irregularly shaped particles characterized by an average size of 33 ± 0.3 um while the result of the X-ray diffraction analysis estimated the crystallinity index (CI) as 0.67 degree of crystallinity, thus, the proportion of crystallinity in KT a-cellulose and 35% of the particles as amorphous in structure. KT a-cellulose, therefore has great potential for use in the food, pharmaceutical and allied industries and is recommended as a way to promote creativity by turning wastes into wealth.


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Department of Chemistry, Alex Ekwueme Federal University, Ndufu-Alike, Nigeria
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