Effect of different nitrogen concentration on the growth, proximate and biochemical composition of freshwater microalgae Monoraphidium contortum
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The objective of this study was to examine the effect of different nitrogen concentrations on the growth, pigments, and proximate composition of freshwater microalgae Monoraphidium contortum. It was observed that higher nitrogen concentration had a significant (p<0.05) impact on enhancing microalgal growth, photosynthetic activity, and protein and carbohydrate content. Maximum cell density (7.19Γ107 cells/mL), dry biomass (0.61g/L), and total chlorophyll (17.42 mg/L) were obtained in the highest concentration of (18.5g/500mL) NaNO3; on the contrary, minimum values were found in 6.5g/500mL NaNO3. Carotenoid (6.14 mg/L) and total phycobiliprotein (4.11 mg/g) were the maximum in control concentrations (12.5g/500mL) of NaNO3. The proximate composition also varied significantly (p<0.05) among all the treatments, where maximum protein (23.77% dry weight) and carbohydrate (22.79% dry weight) were produced in the highest nitrogen concentration (18.5g/500mL NaNO3) and minimum protein (16.36% dry weight) and carbohydrate (14.08% dry weight) were found in the lowest nitrogen concentration (6.5g/500mL NaNO3). Moreover, the highest lipid accumulation (20.07% dry weight) was obtained at the concentration of 6.5g/500mL NaNO3, whereas the lowest 15.88% dry weight lipid was in 12.5g/500mL NaNO3. This study stated that higher nitrogen concentration boosts the growth and nutritional profile of M. contortum, but lower nitrogen concentration enriches lipid production which will be economic in commercial microalgae culture.
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<journal-id journal-id-type="publisher">JIAR</journal-id>
<journal-id journal-id-type="nlm-ta">Journ of innovation in applied research</journal-id>
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<issn pub-type="ppub">2231-2196</issn>
<issn pub-type="opub">0975-5241</issn>
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<publisher-name>Radiance Research Academy</publisher-name>
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<article-id pub-id-type="publisher-id">113</article-id>
<article-id pub-id-type="doi">10.51323/JIAR.10.11.2022.1-12</article-id>
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<subject>Applied Microbiology</subject>
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<article-title>Effect of different nitrogen concentration on the growth, proximate and biochemical composition of freshwater microalgae Monoraphidium contortum
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<contrib contrib-type="author">
<name>
<surname>Mukta</surname>
<given-names>Fardous Ara</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Khatoon</surname>
<given-names>Helena</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Rahman</surname>
<given-names>Mohammad Redwanur</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Acharjee</surname>
<given-names>Mahima Ranjan</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Newase</surname>
<given-names>Subeda</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Nayma</surname>
<given-names>Zannatul</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Sultana</surname>
<given-names>Razia</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Khan</surname>
<given-names>Mohammed Nurul Absar</given-names>
</name>
</contrib>
<contrib contrib-type="author">
<name>
<surname>Hasan</surname>
<given-names>Shanur Jahedul</given-names>
</name>
</contrib>
</contrib-group>
<pub-date pub-type="ppub">
<day>10</day>
<month>11</month>
<year>2022</year>
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<volume>6</volume>
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<fpage>1</fpage>
<lpage>12</lpage>
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<license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.</license-p>
</license>
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<abstract>
<p>The objective of this study was to examine the effect of different nitrogen concentrations on the growth, pigments, and proximate composition of freshwater microalgae Monoraphidium contortum. It was observed that higher nitrogen concentration had a significant (p</p>
</abstract>
<kwd-group>
<kwd>Monoraphidium contortum</kwd>
<kwd> Nitrogen stress</kwd>
<kwd> Growth curve</kwd>
<kwd> Pigments</kwd>
<kwd> Proximate composition </kwd>
</kwd-group>
</article-meta>
</front>
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An, M., Gao, L., Zhao, W., Chen, W., & Li, M. (2020). Effects of nitrogen forms and supply mode on lipid. Energies, 13 (3), 691-697.
Benemann, J.R., & Tillett, D.M. (1987). Microalgae lipid production. Energy from biomass and waste XI. Conference Proceeding, Institute of Gas Technology.
Bligh, E.G., & Dyer, W.J. (1959). A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology, 37 (8), 911-917.
Brow, M.R. (2002). Nutritional value of microalgae for aquculture. In: Avances en NutriciónAcuícola VI. Memorias del VI Simposium Internacional de NutriciónAcuícola. Cruz-Suárez LE, Ricque-Marie D, Tapia- Salazar M, Gaxiola-Cortés MG, Simoes N, editors. Cancún, Quintana Roo, México.
Chisti, Y. (2008). Biodiesel from microalgae beats bioethanol. Trends in Biotechnology, 26 (3), 126-133.
Chu, et al. (2013). Phosphorus plays an important role in enhancing biodiesel productivity of Chlorella vulgaris under nitrogen deficiency. Bioresource Technology, 134 (1), 341346.
Cuellar-Bermudez, et al. (2015). Extraction and purification of high-value metabolites from microalgae: essential lipids, astaxanthin and phycobiliproteins. Microbial Biotechnology, 8 (2), 190209.
Dhup, S., & Dhawan, V. (2014). Effect of nitrogen concentration on lipid productivity and fatty acid composition of Monoraphidium sp. Bioresource Technology, 152, 572-575.
Dubois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28 (3), 350- 356.
Fujii, et al. (2010). Potential use of the astaxanthin-producing microalga, Monoraphidium sp. GK12, as a functional aquafeed for prawns. Journal of Applied Phycology, 22, 363369.
Green, F.B., Lundquist, T., & Oswald, W. (1995). Energetics of advanced integrated wastewater pond systems. Water Science and Technology, 31 (12), 9-20.
Guil-Guerrero, J.L., Navarro-Juarez, R., Lopez-Martinez, J.C., Campra-Madrid, P., & Rebolloso-Fuentes, M.M. (2004). Functionnal properties of the biomass of three microalgal species. Journal of Food Engineering, 65 (4), 511517.
Jeffrey, S.W., & Humphrey, G.F. (1975). New spectrophotometric equations for determining chlorphylls a, b, and c, in higher plants, algae and natural phytoplankton. Biochemie und Physiologie der Pflanzen, 167 (2), 191 -194.
Jenkins, S.H. (1982). Standard methods for the examination of water and wastewater. Water Research, 16 (10), 1495-1496.
Jia, et al. (2015). Molecular mechanisms for photosynthetic carbon partitioning into storage neutral lipids in Nannochloropsis oceanica under nitrogen-depletion conditions. Algal Research, 7 (1), 6677.
Juneja, A., Ceballos, M.R., & Murthy, S.G. (2013). Effects of environmental factors and nutrient availability on the biochemical composition of algae for biofuels production: a review. Energies, 6 (9), 4607?4638.
Kend?rl?oglu, G., Ag?rman, N., & Cet?n, A.K. (2015). The effects of photoperiod on the growth, protein amount and pigment content of Chlorella vulgaris. Turkish Journal of Science and Technology, 10 (2), 7-10.
Khatoon, H., Rahman, N., Suleiman, S., Banerjee, S., & Abol-Munafi, A. (2017). Growth and proximate composition of Scenedesmus obliquus and Selenastrum bibraianum cultured in different media and condition. Proceedings of the National Academy of Sciences, India. Section B: Biological Sciences, 89 (37).
Khatoon, H., Yuan, G.T.G., Mahmud, A.I., & Rahman, M.R. (2020). Growth and carotenoid production of Dunaliella salina (Dunal) teodoresco, 1905 cultured at different salinities. Asian Fisheries Science, 33 (1), 207212.
Lavens, P., & Sorgeloos, P. (1996). Manual on the production and use of live food for aquaculture. Food and Agriculture Organization of the United Nations, Rome.
Leckie, E. (2021). Adelaide scientists turn marine microalgae into superfoods to substitute animal proteins. ABC News, Australian Broadcasting Corporation.
Li, T., Wan, L., Li, A., & Zhang, C. (2013). Responses in growth, lipid accumulation, and fatty acid composition of four oleaginous microalgae to different nitrogen sources and concentrations. Chinese Journal of Oceanology and Limnology, 31(1), 13061314.
Li, Y., Fei, X., & Deng, X. (2012). Novel molecular insights into nitrogen starvation induced triacylglycerols accumulation revealed by differential gene expression analysis in green algae Micractinium pusillum. Biomass Bioenergy, 42 (3), 199-211.
Li, Y., Han, D., Sommerfeld, M., & Hu, Q. (2011). Photosynthetic carbon partitioning and lipid production in the oleaginous microalga Pseudochlorococcum sp. (Chlorophyceae) under nitrogen?limited conditions. Bioresource Technology, 102 (1), 123?129.
Lin, et al. (2019). Monoraphidium sp. HDMA-20 is a new potential source of α-linolenic acid and eicosatetraenoic acid. Lipids in Health and Disease, 18(1), 5665.
Lowry, O.H., Rosebrough, N.J., Farr, A.L., & Randall, R.J. (1951). Protein measurement with the folin phenol reagent. Journal of Biological Chemistry, 193 (1), 265-275.
Menegol, T., Diprat, A.B., Rodrigues, E., & Rech, R. (2017). Effect of temperature and nitrogen concentration on biomass composition of Heterochlorella luteoviridis. Food Science and Technology, 37 (3), 28-37.
Mishra, U. & Pabbi, S. (2004). Cyanobacteria: a potential biofertilizer for rice. Resonance, 9 (1), 6- 10.
Nadzir, S.M., Yusof, N., Nordin, N., Kamari, A., & Yusoff, M.Z.M. (2021). Production of lipid and carbohydrate in Tetradesmus obliquus UPSI-JRM02 under nitrogen stress condition. Jurnal Teknologi, 83 (2), 27-35.
Nayak, M., Suh, W.I., Chang, Y.K., & Lee, B. (2019). Exploration of two-stage cultivation strategies using nitrogen starvation to maximize the lipid productivity in Chlorella sp. HS2. Bioresource Technology, 276 (4), 110-118.
Podevin, M., De-Francisci, D., Holdt, S.L., & Angelidaki, I. (2015). Effect of nitrogen source and acclimatization on specific growth rates of microalgae determined by a high-throughput in vivo microplate autofluorescence method. Journal of Applied Phycology, 27 (4), 14151423.
Pruvost, J., van Vooren, G., le Gouic, B., Couzinet?Mossion, A., & Legrand, J. (2011). Systematic investigation of biomass and lipid productivity by microalgae in photobioreactors for biodiesel application. Bioresource Technology, 102 (1), 150?158.
Qu, et al. (2019). Comparison of monoculture and mixed culture (Scenedesmus obliquus and wild algae) for C, N, and P removal and lipid production. Environmental Science and Pollution Research, 26 (20), 2096120968.
Ratha, et al. (2016). A rapid and reliable method for estimating microalgal biomass using a moisture analyser. Journal of Applied Phycology, 28 (1), 1725-1734.
Saha, et al. (2013). Effect of various stress-regulatory factors on biomass and lipid production in microalga Haematococcus pluvialis. Bioresource Technology, 128, 118-124.
Saha, S.K., Uma, L., & Subramanian, G. (2003). Nitrogen stress induced changes in the marine cyanobacterium Oscillatoria willei BDU 130511. FEMS Microbiology Ecology, 45 (3), 263?272.
Siegelman, H., & Kycia, J. (1978). Algal bili-proteins: Handbook of psychological method. Cambridge University Press, Cambridge. p. 71-79.
Silveira, S.T., Burkert, J.F.M., Costa, J.A.V., Burkert, C.A.V., & Kalil, S.J. (2007). Optimization of phycocyanin extraction from Spirulina platensis using factorial design. Bioresource Technology, 98 (8), 1629-1634.
Simionato, et al. (2013). The response of Nannochloropsis gaditana to nitrogen starvation includes de novo biosynthesis of triacylglycerols, a decrease of chloroplast galactolipids, and reorganization of the photosynthetic apparatus. Eukaryotic Cell, 12 (5), 665676.
Singh, R., Paliwal, C., Nesamma, A.A., Narula, A., & Jutur, P.P. (2020). Nutrient deprivation mobilizes the production of unique tocopherols as a stress-promoting response in a new indigenous isolate Monoraphidium sp. Frontiers in Marine Science, 7, 2296-2305.
Soletto, D., Binaghi, L., Lodi, A., Carvalho, J.C.M., & Converti, A. (2005). Batch and fed-batch cultivations of Spirulina platensis using ammonium sulphate and urea as nitrogen sources. Aquaculture, 243 (1-4), 217224.
Stein, J.R., editor. (1980). Handbook of phycological methods: Culture methods and growth measurements. Cambridge University Press, Cambridge. pp. 448.
Toyub, M., Miah, M., Habib, M., & Rahman, M. (2008). Growth performance and nutritional value of Scenedesmus obliquus cultured in different concentrations of sweetmeat factory waste media. Bangladesh Journal of Animal Science, 37 (1), 86- 93.
Ugwu, C.U., Aoyagi, H., & Uchiyama, H. (2008). Photobioreactors for mass cultivation of algae. Bioresource Technology, 99 (10), 4021-4028.
Wijesekara, I., Pangestuti, R., & Kim, S.K. (2010). Biological activities and potential health benefits of sulfated polysaccharides derived from marine algae. Carbohydrate Polymers, 84 (1), 14- 21.
Xie, T., Xia, Y., Zeng, Y., Li, X., & Zhang, Y. (2017). Nitrate concentration-shift cultivation to enhance protein content of heterotrophic microalga Chlorella vulgaris: over- compensation strategy. Bioresource Technology, 233, 247255.
Xu, Y., Ibrahim, M.I., & Harvey, J.P. (2016). The influence of photoperiod and light intensity on the growth and photosynthesis of Dunaliella salina (chlorophyta) CCAP 19/30. Plant Physiology and Biochemistry, 106, 305315.
Yang, et al. (2008). Growth and lipid accumulation by different nutrients in the microalga Chlamydomonas reinhardtii. Biotechnology for Biofuels, 11 (1), 40-48.
Yu, et al. (2012). Isolation of a novel strain of Monoraphidium sp. and characterization of its potential application as biodiesel feedstock. Bioresource Technology, 121, 256262.
Zarrinmehr, et al. (2020). Effect of nitrogen concentration on the growth rate and biochemical composition of the microalga Isochrysis galbana. Egyptian Journal of Aquatic Research, 46 (1), 153-158.
Zhang, L., & Liu, J. (2016). Enhanced fatty acid accumulation in Isochrysis galbana by inhibition of the mitochondrial alternative oxidase pathway under nitrogen deprivation. Bioresource Technology, 211, 783786.
Zhao, et al. (2017). Nitrogen starvation impacts the photosynthetic performance of Porphyridium cruentum as revealed by chlorophyll a fluorescence. Scientific Reports, 7 (1), 8542-8546.
Zhu, S., Huang, W., Xu, J., Wang, Z., & Yuan, Z. (2014). Metabolic changes of starch and lipid triggered by nitrogen starvation in the microalgae Chlorella zofingiensis. Bioresource Technology, 152: 292298.
Department of Aquaculture, Faculty of Fisheries, Chattogram Veterinary and Animal Sciences University, Chattogram, Bangladesh
Department of Fishing and Post-Harvest Technology, Chattogram Veterinary and Animal Sciences University