Comparative study of growth, pigments and proximate composition of selected indigenous freshwater microalgae isolated from Bangladesh
Microalgae are considered as natural producers of protein, lipid, carotenoids, and fatty acids that are important not only in human and animal nutrition but also in medicines. Selection of the best performing locally isolated strains should be carried out to assess the nutritional properties of them as they are well adapted to local environment and exhibit better performance. Therefore, in the present study four independent primordial freshwater microalgae (Selenastrum sp., Ankistrodesmus sp., Monoraphidium sp., and Scenedesmus sp.) isolated from Bangladesh, were used to determine their growth, pigments and proximate composition. For growth determination, data were collected in terms of cell density and optical density, mass cultured in commercial Bold Basal Media and harvested at their stationary phases. Results showed that, onset of stationary phase (9-14 days) varied among the four species where Selenastrum sp. showed significantly highest cell density (5.66±0.08cells/mlx107). Among the four species Monoraphidium sp. showed significantly highest (p<0.05) chlorophyll a (11.264±0.065μg/mL), b (2.082±0.067μg/mL) and carotenoid content (8.05±0.07μg/mL). Moreover, Total phycobiliproteins was highest (p<0.05) in Scenedesmus sp. (6.105±0.12mg/g). Protein (32.44±2.17%dry weight) and lipids (25.28±0.31 %dry weight) content were significantly (p<0.05) highest in Monoraphidium sp. and Carbohydrates in Scenedesmus sp. (21.06±0.19 % dry weight). Based on the obtained results, it was concluded that, Monoraphidium sp. can be utilized as potential pigments source and Monoraphidium sp. and Selenastrum sp. as feed source to utilize those in different commercial application.
<?xml version="1.0" encoding="UTF-8" standalone="yes"?> <!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2d1 20170631//EN" "JATS-journalpublishing1.dtd"> <article xlink="http://www.w3.org/1999/xlink" dtd-version="1.0" article-type="applied-microbiology" lang="en"> <front> <journal-meta> <journal-id journal-id-type="publisher">JIAR</journal-id> <journal-id journal-id-type="nlm-ta">Journ of innovation in applied research</journal-id> <journal-title-group> <journal-title>Journal of Innovation in Applied Research</journal-title> <abbrev-journal-title abbrev-type="pubmed">Journ of innovation in applied research</abbrev-journal-title> </journal-title-group> <issn pub-type="ppub">2231-2196</issn> <issn pub-type="opub">0975-5241</issn> <publisher> <publisher-name>Radiance Research Academy</publisher-name> </publisher> </journal-meta> <article-meta> <article-id pub-id-type="publisher-id">105</article-id> <article-id pub-id-type="doi">10.51323/JIAR.5.1.2022.13-24</article-id> <article-id pub-id-type="doi-url"/> <article-categories> <subj-group subj-group-type="heading"> <subject>Applied Microbiology</subject> </subj-group> </article-categories> <title-group> <article-title>Comparative study of growth, pigments and proximate composition of selected indigenous freshwater microalgae isolated from Bangladesh </article-title> </title-group> <contrib-group> <contrib contrib-type="author"> <name> <surname>Nayma</surname> <given-names>Zannatul</given-names> </name> </contrib> <contrib contrib-type="author"> <name> <surname>Khatoon</surname> <given-names>Helena</given-names> </name> </contrib> <contrib contrib-type="author"> <name> <surname>Rahman</surname> <given-names>Mohammad Redwanur</given-names> </name> </contrib> <contrib contrib-type="author"> <name> <surname>Mukta</surname> <given-names>Fardous Ara</given-names> </name> </contrib> <contrib contrib-type="author"> <name> <surname>Sultana</surname> <given-names>Razia</given-names> </name> </contrib> <contrib contrib-type="author"> <name> <surname>Nuruzzaman</surname> <given-names>Mohammad</given-names> </name> </contrib> </contrib-group> <pub-date pub-type="ppub"> <day>27</day> <month>04</month> <year>2022</year> </pub-date> <volume>5</volume> <issue/> <fpage>13</fpage> <lpage>24</lpage> <permissions> <license license-type="open-access" href="http://creativecommons.org/licenses/by/4.0/"> <license-p>This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.</license-p> </license> </permissions> <abstract> <p>Microalgae are considered as natural producers of protein, lipid, carotenoids, and fatty acids that are important not only in human and animal nutrition but also in medicines. Selection of the best performing locally isolated strains should be carried out to assess the nutritional properties of them as they are well adapted to local environment and exhibit better performance. Therefore, in the present study four independent primordial freshwater microalgae (Selenastrum sp., Ankistrodesmus sp., Monoraphidium sp., and Scenedesmus sp.) isolated from Bangladesh, were used to determine their growth, pigments and proximate composition. For growth determination, data were collected in terms of cell density and optical density, mass cultured in commercial Bold Basal Media and harvested at their stationary phases. Results showed that, onset of stationary phase (9-14 days) varied among the four species where Selenastrum sp. showed significantly highest cell density (5.66±0.08cells/mlx107). Among the four species Monoraphidium sp. showed significantly highest (p</p> </abstract> <kwd-group> <kwd>Stationary phase</kwd> <kwd> microalgae</kwd> <kwd> growth</kwd> <kwd> pigments</kwd> <kwd> proximate composition</kwd> </kwd-group> </article-meta> </front> </article>
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