PREVALENCE AND CHARACTERIZATION OF WATERBORNE MULTI DRUG RESISTANT E. COLI O157:H7

Category: Previous Issue Created: Friday, 27 January 2017 Published: Friday, 27 January 2017 Written by Kamlesh Choure* and Shilpi Singh, Anita Bagri, Shivpal Verma

 

Department of Biotechnology, Faculty of Life Sciences and Technology, AKS University, Satna (M.P.) 485001

 

*Corresponding author : Shilpi Singh, Anita Bagri, Shivpal Verma and Kamlesh Choure*

 

*Corresponding author E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

 

 

 

ABSTRACT

The water samples were collected from pre-identified spots. To isolate E. coli from the water samples a number of nutrient media with selective substrates were used. MacConkey agar plates are used to differentiate by fermenting lactose. Lactose fermenting strains which grew as red or pink. MUG-EC broth was used for in the E. coli strains. Lastly, confirmation of selected suspected E. coli 0157:H7 isolates were taken to test for indole production test and methyl red red. Four sampling sites S1, S2, S5 & S6 were found positive for the presence of E. coli 0157:H7. Isolated virulent bacteria were further characterized based on the presence of stx2 gene that codes for shiga like toxins. The product targeting Stx2 of 485 bp was found in all suspected E. coli isolates from the sampling site S1, S2, S3 & S6. This four E. coli strains possessed virulence marker Stx–2 gene as tested by PCR. Phylogenetic relationship among isolated bacteria was determined by the sequence analysis of 16S rDNA gene. The specific oligomers target 16S RNA sequence of E. coli and 100% sequence similarity was found with the reference sequence after BLAST analysis of most conserved region of 16S r DNA gene. Antimicrobial susceptibility testing revealed the multi drug resistant pattern of isolates. All E. coli isolates were resistant to ampicillin, 6 isolates were resistant to erythromycin, 4 isolates were resistant to tetracyclin, and 2 isolates were resistant to kanamycin.

 

 

Download the pdf Visit and view full articles in PDF

 

 

Hits: 1337